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Peer Reviewed Study Showing Specific EBV Prot

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Technophobe View Drop Down
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    Posted: May 09 2019 at 2:09am
Ebola virus VP35 has novel NTPase and helicase-like activities
Ting Shu Tianyu Gan Peng Bai Xiaotong Wang Qi Qian Hui Zhou Qi Cheng Yang Qiu Lei Yin Jin Zhong


Nucleic Acids Research, gkz340, https://doi.org/10.1093/nar/gkz340
Published:
08 May 2019
Abstract

Ebola virus (EBOV) is a non-segmented, negative-sense RNA virus (NNSV) in the family Filoviridae, and is recognized as one of the most lethal pathogens in the planet. For RNA viruses, cellular or virus-encoded RNA helicases play pivotal roles in viral life cycles by remodelling viral RNA structures and/or unwinding viral dsRNA produced during replication. However, no helicase or helicase-like activity has ever been found to associate with any NNSV-encoded proteins, and it is unknown whether the replication of NNSVs requires the participation of any viral or cellular helicase. Here, we show that despite of containing no conserved NTPase/helicase motifs, EBOV VP35 possesses the NTPase and helicase-like activities that can hydrolyse all types of NTPs and unwind RNA helices in an NTP-dependent manner, respectively. Moreover, guanidine hydrochloride, an FDA-approved compound and inhibitor of certain viral helicases, inhibited the NTPase and helicase-like activities of VP35 as well as the replication/transcription of an EBOV minigenome replicon in cells, highlighting the importance of VP35 helicase-like activity during EBOV life cycle. Together, our findings provide the first demonstration of the NTPase/helicase-like activity encoded by EBOV, and would foster our understanding of EBOV and NNSVs.

Full article: https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkz340/5486747
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Post Options Post Options   Thanks (0) Thanks(0)   Quote FluMom Quote  Post ReplyReply Direct Link To This Post Posted: May 09 2019 at 7:07pm
Ok so in regular English what does this mean? It can't be cured? Sorry I am not smart enough to understand all of this.
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Post Options Post Options   Thanks (0) Thanks(0)   Quote Technophobe Quote  Post ReplyReply Direct Link To This Post Posted: May 10 2019 at 2:34am
You are not alone there, FluMom.

Basically, it gives a few more details of the DNA (RNA in this case) building/demolishing machines that control the virus' reproductive processes (the hands that unzip the DNA zipper so each half can then make a copy of itself).

Those searching for treatments and vaccines can use that to their advantage. Up until now the 'hands' have been invisible in this virus. But in some viruses the hands can be disabled. This is the method by which some antivirals work.   

Those looking for the origins of the virus can also possibly squeeze some info out of this too.
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Post Options Post Options   Thanks (0) Thanks(0)   Quote Technophobe Quote  Post ReplyReply Direct Link To This Post Posted: May 10 2019 at 2:51am
You can get a nice explanation of helicases from Wikipedia here https://en.wikipedia.org/wiki/Helicase

Wikipedia also has a good article on negative-sense viuruses herehttps://en.wikipedia.org/wiki/Negative-sense_single-stranded_RNA_virus

You can't always trust Wikipedia, as some of the researchers/updaters have nasty senses of humour. But it is a great jumping-off point for further research. These two articles are both fine by the way.
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