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Scared Straight

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Printed Date: April 18 2024 at 3:58am


Topic: Scared Straight
Posted By: maryk
Subject: Scared Straight
Date Posted: March 28 2006 at 9:47pm

Scared straight.

I went to the store after I got off work tonight. I usually go on my day’s off with my husband, thought I would get a jump on things. Not a lot of people in their this time of night, so I was surprised when this man stopped to talk to me. He said “hi“, and I turned to look at him and replied back. At the same time I said hello I looked down and saw a basket full of chicken , I mean all most up to the top of the cart. I did not think BF, I thought Barbecue , so I said “looks like some one might be enjoying the nice weather with a barbecue. “no” he said “bird flu“. “I’m stocking up“. I said cool and started walking away. He started following me and said “ ya do you know about the BF“, I said “ ya I have heard. Stocking up may help prevent some death if we get quarantined“. He said no I am not saving the chicken I am eating it now, “OK?“ I said ( a weird guy) kept walking(wishing I had my husband their with me) The guy started saying he wanted to catch the BF. That he had a stock pile of Ramify and he wanted to be the first person to get it so he could make sure it got passed around to prove to the world our government can’t handle sh*t. Then he pulled this vile out as he was explaining that it contained the regular flu virus. I left as fast as I could, just started for the door.




Replies:
Posted By: Guests
Date Posted: March 28 2006 at 9:50pm
Wow, I'd probably have to hit that guy. Was he big? Was there a shovel nearby? We don't need people like that in the world.


Posted By: Guests
Date Posted: March 28 2006 at 9:50pm
I asked Mary to post this in both the News room and the General Discussion room. 
 
This is truly disturbing!!!  Not that I think for a second that it would work, but that there is a nut out there thinking about it and formulating a plan, and acting on said plan.   I'm just grateful he's stupid.  But what if there is someone out there, or several others, or many others with the same thought.
 
Of course, we can also hope that he was just a nut trying to scare someone.


Posted By: maryk
Date Posted: March 28 2006 at 9:56pm
major serial killer. can this be done?


Posted By: Siameselade
Date Posted: March 28 2006 at 9:57pm
Dead More of them this one is enough for me. Call the looney bin!!

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Siam


Posted By: Guests
Date Posted: March 28 2006 at 10:39pm
You're american, don't you have like 3 guns on you at all times? LOL You could have just gone *blam* *blam* and solved that problem.


Posted By: maryk
Date Posted: March 28 2006 at 10:43pm
Their is no way, Then i would have worry about BF from a Prisoners persecutive. It's not all out their yet.


Posted By: Guests
Date Posted: March 28 2006 at 10:44pm
You know.... I was gonna make a joke about "Anybody seen Corn lately" but decided that humor wasn't appropriate to the thread.  But since David did it...
 
Anybody seen Corn lately?
 
Seriously, I have PM'ed Maryk and asked her to take this to the authorities.  This nut needs to be checked out at a minimum and maybe figuratively slapped up side the head.
 
SZ


Posted By: maryk
Date Posted: March 28 2006 at 10:48pm
I think that can be done. I will go to the Police tomorrow. I have written down what i can remember. I am sure someone had to notice a basket full of chicken. Maybe someone knows him.


Posted By: Guests
Date Posted: March 28 2006 at 10:50pm
I wonder if he followed the saying "don't put all your influenza in one basket"LOL


Posted By: Guests
Date Posted: March 28 2006 at 11:46pm
MaryK...Most groceries have video surveillance camera's (they do it for theft prevention) if you can give as many details as you can, they can pick up the rest.  Key is not to wait 'cause they often reuse tape.

We always looking out for that one crazy guy to do something really nuts.  "The one crazy guy" with a plan is a reality and I am sure there are many in his category.  The police and the FBI deal with them all the time.

Not sure if you called the store as soon as you got yourself to safety, but that is a great thing to do.  If you can't reach the store, that type of situation is ok for 911 --you have no idea what he had in that vial.  He may have been a run of the mill crazy with some colored water or somebody serious with a pathogen.  The cops can sort it out.  Key thing is get safe first, then call.

What an experience!!!!


Posted By: Guests
Date Posted: March 29 2006 at 12:35am
The man is insane.
 
First, no sane person would volunteer to die such a horrible death as drowning in your own fluids just from h5n1 alone. And volunteering to be Patient Zero for the Pandemic strain? That is just nuts.
 
Second, he wants to prove that the goverment cant handle it? The government already is openly admitting that they cant, they are telling people to prepare and being vocal about telling us we are own our own if Pandemic strkes. His logic is flawed.
 
Sadly here is a man who does not love one single person on earth. If he did, he would do everything in his power to be prepared.
 
And the fact that he would walk up to a total stranger and disclose his diabolical plan just shows that he is a man in crisis, on edge, reaching out, just hoping someone would stop him. Think about it... if you really wanted to do this, would you go around telling strangers about it? You wouldn't, you would just do it. This man on some subconscious level wants to be stopped.
 
Here is a man with no hope, with no love, and is desperate. It is possible that the vial contained nothing dangerous and he is just desperately reaching out (hopefully!). Either way, he has displayed that he is suicidial, potentially homocidal, and needs help. Report him to the authorities, and hopefully they can find him get him the psychological help he needs.


Posted By: Guests
Date Posted: March 29 2006 at 1:23am
You should have told him not only will he prove our government is inept he will do his small part, as he assumes room temperature, to halt global warming, cut our dependence on fossil fuels and reduce the overpopulation "crisis".


Posted By: sweets
Date Posted: March 29 2006 at 1:25am
What has happened to this country? It just gets worse and worse. The amount of nutters is greater than the sane these days

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I'm not a ribbering Jidiot!
www.exaltedshrimp.com


Posted By: Amethyst
Date Posted: March 29 2006 at 5:17am

Sounds like that guy was probably mentally ill.  At least he was buying chickens and not going around shooting people.

He probably doesn't even realize that cooking the chickens kills the virus, and not cooking them will give him a really bad case of salmonella poisoning, not the bird flu.

 

 



Posted By: Guests
Date Posted: March 29 2006 at 6:57am
'Oh No!', said Chicken Licken, 'the sky is falling down and I'm on my way to tell the king!'. So Chicken Licken went on his way to tell the King.

On his way, Chicken Licken met Henny Penny.

'Oh! Henny Penny', said Chicken Licken, 'the sky is falling down and I'm on my way to tell the King'.

'Oh No!', said Henny Penny, 'then I'd better come with you".

So Henny Penny and Chicken Licken went on their way to tell the King............


Posted By: JaxMax
Date Posted: March 29 2006 at 7:12am
Justme-
 
18 men were found willing to burn to death crashing into the World Trade Center on 911. The US military discusses Pandemic Flu as a weapon  at length in their deployment website-"Pandemic Flu as a suicide attack" in response to questions from serviemembers.


-------------
He who walks with the wise grows wise, but a companion of fools suffers harm.Proverbs 13:20, The Bible


Posted By: Left Field
Date Posted: March 29 2006 at 7:42am
JaxMax you're right on target. 


Posted By: maryk
Date Posted: March 29 2006 at 7:59am

Went to the police station this morning. I gave them my statement. Apparently they went to the store last night and questioned the employs who were their ( they were upset that i left a large cart of food for them to put away) Yes they knew the man, a regular in the store. owns a lawn maintenance company. Police went to talk to him. They said he thought i was cute and was just trying to make small talk, said he knew nothing of the BF. Chicken is for a crew party this weekend. he is single and often has the crew and their family over.

I felt like they thought i was the nut case, suggested i see my doctor. I may be stressing my self out with all this talk of BF


Posted By: maryk
Date Posted: March 29 2006 at 8:13am

As i sit here and think about what happened last night i start to wonder how many people out their who could be thinking along the same lines as this guy. Maybe he was just getting his kicks, like scaring people gets him off, or maybe he really wants to see the Bf erupt into a major killing machine. Can any person who may come in contact with the BF make this mutation happen to where it can easily transmit H2H?



Posted By: Guests
Date Posted: March 29 2006 at 8:17am
When you think of all those men who have unprotected sex when they are hiv positive reckles .  I beleive you arent under stress,    I bet the people who work with him think he is a jerk.  
 
 


Posted By: araywood
Date Posted: March 29 2006 at 8:28am

I am sure Hamas, Taliban and Al queda are already working on it. Soon they will be driving around with culled chickens in there cars instead of explosives.

 Whether the cops thought you were crazy or not they know who this guy is. Thats the most important thing. You did your community a service appreciated or not. What if he wasn't kidding?????


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NO NEWS IS WHO NEWS


Posted By: mondegreen
Date Posted: March 29 2006 at 9:05am

Dear Scared Straight

Going to the police is the wrong office, you need to contact the Joint Terrorism Task Force in your area. They are the only org given authority to investigate such things as you stated. Just the fact he stated he wanted to spread the H5 and as you stated held up a vial of something. Maybe he is a quack but he is also a dangerous person. Contact your local joint terrorism task force immediately for your own sake.If you have problems finding the nbr I will place you in contact with them.



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A word when spoken is misunderstood!


Posted By: araywood
Date Posted: March 29 2006 at 9:09am
Homeland security?. Jese what a great pick up line. "Hey baby I am going to infect the world" MOOHOHAAA. Real ladie man. I'll have to try that one.Wacko 

-------------
NO NEWS IS WHO NEWS


Posted By: Guests
Date Posted: March 29 2006 at 9:16am
Originally posted by JaxMax JaxMax wrote:

Justme-
 
18 men were found willing to burn to death crashing into the World Trade center on 911. The US military discusses Pandemic Flu as a weapon  at length in their deployment website-"Pandemic Flu as a suicide attack" in response to questions from serviemembers.
 
Yes, and they **rtainly did not go walking around telling strangers about it beforehand. I do not doubt that there are people in the world who would do such a thing. I just think that if this man had been a terrorist, he would have been a seriously inept one. Terrorists dont go around telling strangers their plans. If he was serious about what he was saying, then it seems that he was reaching out for help. The fact that he could joke about something like this, well, then he is just a jerk.


Posted By: maryk
Date Posted: March 29 2006 at 9:36am
Terrorism Task For** in my area will first contact the poli** for all information pertaining to this event. and the poli** will surly tell them I'm just a stressed out BF nut.


Posted By: Guests
Date Posted: March 29 2006 at 9:39am
mondegreen is absolutely right - you should contact Joint Terrorism Task Force - your local police force is inept.

I can't believe that they think this was small talk.  What was it "Together we could infect the world?"

If he thought it was a joke, he should be informed that it was a terrorist threat, and your local police force should have a refresher on terrorism. You are owed an apology as well.   geeeeeeeze!


Posted By: maryk
Date Posted: March 29 2006 at 9:50am
OK i Have a number. Going to go call. Will get back with you all latter. Thanks for all your support. I am still shaken up over this.


Posted By: spread_fear
Date Posted: March 29 2006 at 10:02am
Mary K, this guy knows about you.  People dont come out and say things like that without motive.  I suspect the Federal Government my be involed with this guy and based on the situation you described, he knew something.  You were alone which is the beginning factior.  He knew to target you with specific information about the bird flu.  I would watch and see if this guy re-emerges some where.  Look for suspicious vehicles parked in front of your house, particularly at night.  I am not trying to scare you but make you alert to the fact you may be targeted by the feds.  Do not go to the authorities yet unless you can prove something and even then, be weary.  I immediatly raised red flags as soon as I read your post.  Be careful about your phone conversations and internet chatter.  Drop some bait and see if you get a reaction.  I dont think he is a regular nut, he was specific.  You need to talk to your husband about some house hold security measures and implement them.  I really, honestly think you are on the F.B.I. watch list if this is the case.  Either way, nut or fed, be CAREFUL.  Carry a weapon and a tape recorder.  Go alone to other public areas where there is a lot of people around and let them come to you, get them on tape and keep records of these incedences.  Take action to see if this is a one time crazy guy fluke or if there are reoccurances.  Dont take my advice to heart or go crazy about it but be cautious.


Posted By: cv1632
Date Posted: March 29 2006 at 10:17am
Unfortunately, there are a whole lot more people out there that would like to destroy our country in any way possible than we would like to admit.

Some of them are already locked up, but Im afraid that most are not. Some people would do anything to destroy life as we know it.

It really doesnt matter if they are insane or not if they get the job done that they wanted to do.
    

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Why do some people Hate, with incredible passion, and yet without reason, consideration of fact, or any allowance for rebuttal. Father God, forgive me if I should ever be this intolerant of others.


Posted By: Fiddlerdave
Date Posted: March 29 2006 at 10:40am
 
Spread_Fear, this is way off from being a checkup by authorities. I think she probably gave enough signals that Nutcase felt comfortable sharing his issue (BF) which MaryK shares.  Remember, the store employees said he was a regular - any kind of undercover would be new, and the police would not be able go to his house.
 
His message was tailored to get (A) get sympathy (I'm gonna give myself BF and die to save the world!) and (B) get her involved (I have flu RIGHT HERE!).
 
Yes, he's a nutcase.  In particular, a nutcase who'd like some female companionship and/or followers..  No, he is not Feds undercover.  They would make SENSE!  Because the feds would be making it up, and a made up story would made up to make some kind of sense to HER!
 
I suppose Maryk could keep enough lookout to make sure he is not a stalker, but feds, no way.  And if you ever think the FEDS are after you. LEAVE THE GUN AT HOME LOCKED UP.  All you give them is an excuse.


-------------
Dave
"Ask not for whom the bell tolls, it tolls for us"!


Posted By: Guests
Date Posted: March 29 2006 at 2:07pm
MaryK don't let this worry you or make you wonder if you are the one with issues.  This guy sounds bored and slightly disturbed.  If he uses this as a pick-up line, as said, he'll be single for a long time, or end up with a female just as sick as he is.  Hope it's the latter, he deserves it.


Posted By: Guests
Date Posted: March 29 2006 at 2:24pm
A couple of things...
 
1) Maryk did the right thing...IMO.
 
2) While this guy is no doubt a certifiable nut, so was John Wayne Gacy and Jeffrey Dahmer.
 
3) Anyone who dismisses a threat of this nature is not aware that it's not that difficult to do.  I will paste an article that references research that threw the scientific, health, and security agencies around the world into an unprecidented tizzy.  While I believe the original research was eventually yanked from publication, it was out there for a while.  Why were they afraid????  It ain't that hard and reserchers had just published how to do it.
 
4) While a threat of this nature is minute it isn't zero.... again, JMO.
 
http://jvi.asm.org/cgi/content/full/75/18/8353 - http://jvi.asm.org/cgi/content/full/75/18/8353
 
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Journal of Virology, September 2001, p. 8353-8355, Vol. 75, No. 18
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.18.8353-8355.2001
http://jvi.asm.org/misc/terms.shtml - Copyright © 2001 , http://www.asmusa.org/ - American Society for Microbiology . All rights reserved.

MINIREVIEW

Creation of Killer Poxvirus Could Have Been Predicted

Arno Müllbacher http://jvi.asm.org/cgi/content/full/75/18/8353#FN150 - * and Mario Lobigs

Division of Immunology and Cell Biology, John Curtin School of Medical Research, The Australian National University, Canberra City, Australian Capital Territory 2601, Australia


    TEXT
http://jvi.asm.org/cgi/content/full/75/18/8353#Top"> Top
Text
http://jvi.asm.org/cgi/content/full/75/18/8353#References"> References

Genetic engineering of viruses has become a common practice. One major objective is to use engineered viral vectors for the delivery of genetic information with therapeutic intent or to modify viruses so as to alter the host's immune response to it. Changes in virulence and host range of modified viruses are generally hard to predict. One such virus was recently described in an article published in the Journal of Virology ( http://jvi.asm.org/cgi/content/full/75/18/8353#B8 - 8 ) which received wide coverage in both the scientific ( http://jvi.asm.org/cgi/content/full/75/18/8353#B7 - 7 , http://jvi.asm.org/cgi/content/full/75/18/8353#B8 - 8 , http://jvi.asm.org/cgi/content/full/75/18/8353#B15 - 15 ) and popular press, as well as the electronic media.

The reason for the heightened public interest in this publication was the implication that a means had inadvertently been discovered to allow the creation of killer viruses with potential biological warfare application. According to some of the authors of the above study, "this came as a complete surprise and was totally unexpected." However, a closer reading of the literature on the consequences of infection with recombinant poxviruses encoding interleukin-4 (IL-4), some performed by members of the above study or former colleagues, suggests otherwise.

Ectromelia virus (Ect), an orthopoxvirus and close relative of variola virus (smallpox) and vaccinia virus (VV) (the poxvirus used to vaccinate against smallpox), is a natural mouse pathogen and causes mousepox. Virulence depends on the host genetic background and ranges from the highly susceptible A/J strain of mice (50% lethal dose [LD50] of 0.04 PFU) to the relatively resistant C57BL/6 mice (LD50 of >105 PFU) ( http://jvi.asm.org/cgi/content/full/75/18/8353#B5 - 5 ). Recovery from Ect infection in the genetically resistant strain is absolutely dependent on the cytotoxic T (Tc) cell-mediated exocytosis pathway of cytolysis and in particular on the presence of granzymes A and B ( http://jvi.asm.org/cgi/content/full/75/18/8353#B10 - 10 , http://jvi.asm.org/cgi/content/full/75/18/8353#B12 - 12 ). VV, on the other hand, is much less virulent, and only causes mortality in mice and humans in immunocompromized individuals or by using very high doses (>107 PFU) in mice.

VV has become one of the most frequently used vectors for the expression of genes from pathogens and mammalian genes in mammalian cells in vivo and in vitro. To improve or alter the host's immune response to VV itself and or to the products of foreign inserted genes, additional genes coding for cytokines have been added to the VV genome ( http://jvi.asm.org/cgi/content/full/75/18/8353#B14 - 14 ). The rationale behind including IL-4 in such constructs was the assumption that a type 2 cytokine such as IL-4 would skew the immune response to an elevated antibody response at the expense of a Tc cell response. Early work by Andrew and colleagues ( http://jvi.asm.org/cgi/content/full/75/18/8353#B1 - 1 , http://jvi.asm.org/cgi/content/full/75/18/8353#B2 - 2 ) reported a diminished Tc cell response to VV and recombinant influenza virus hemagglutinin (HA) after immunization with VV recombinants encoding IL-4. In addition and contrary to expectations, no enhancement in the antibody response to either VV or antigen HA was observed. Most important is their comment: "however, this paper demonstrates that IL-4 is unlikely to increase vaccine efficacy and for the first time documents that IL-4 can be lethal when administered in vivo" ( http://jvi.asm.org/cgi/content/full/75/18/8353#B1 - 1 ).

Results verifying these initial studies of decreased lytic activity in splenocytes after immunization with VV-IL-4 recombinants have been documented ( http://jvi.asm.org/cgi/content/full/75/18/8353#B4 - 4 , http://jvi.asm.org/cgi/content/full/75/18/8353#B15 - 15 ). A role for IL-4 in downregulation of cytolytic activity of splenocytes is supported by experiments using mice lacking IL-4 expression ( http://jvi.asm.org/cgi/content/full/75/18/8353#B20 - 20 ) or transgenic mice overexpressing IL-4 ( http://jvi.asm.org/cgi/content/full/75/18/8353#B7 - 7 ). The generally accepted interpretation of the mechanism responsible for the reduced observed Tc cell activity was that IL-4 caused immune deviation from a type 1 response to a type 2 response ( http://jvi.asm.org/cgi/content/full/75/18/8353#B6 - 6 ). However, a very recent study by Aung and Graham ( http://jvi.asm.org/cgi/content/full/75/18/8353#B3 - 3 ) suggests that a different mechanism other than immune-class deviation may be involved or at least contribute to the observed diminished Tc cell activity when IL-4 recombinant viruses are used as immunogens. They showed convincingly that rather than diminished Tc cell responses after immunization with VV-IL-4 recombinants, the antigen-specific lysis of target cells was dependent on the expression of Fas on target cells. Thus, it appears that the presence of IL-4 switches the cytolytic mechanism of Tc cells from the exocytosis (perforin and granzyme)-mediated pathway to the Fas/Fas ligand-mediated pathway of target cell death. Thus, cytolytic effector function should theoretically be operative, although it may be delayed. This assumption is based on recent published studies showing that target cells not expressing Fas do become Fas positive in the presence of Tc cells. This mechanism was identified by using Tc cells from perforin-deficient mice ( http://jvi.asm.org/cgi/content/full/75/18/8353#B17 - 17 ).

The observed failure of poxvirus-preimmunized mice to be protected from a subsequent challenge with the Ect-IL-4 recombinant virus ( http://jvi.asm.org/cgi/content/full/75/18/8353#B8 - 8 ) strongly suggests that memory Tc cells are vital for protection from secondary poxvirus infections. In addition, it indicates that activation of memory Tc cell precursors by antigen-presenting cells expressing IL-4 is modulated similarly, as are naive Tc cell precursors.

The caveat in the case of orthopoxvirus infection is that a number of orthopoxviruses have been shown to inhibit Tc cell-mediated killing of target cells, predominantly via the Fas pathway ( http://jvi.asm.org/cgi/content/full/75/18/8353#B9 - 9 , http://jvi.asm.org/cgi/content/full/75/18/8353#B11 - 11 , http://jvi.asm.org/cgi/content/full/75/18/8353#B18 - 18 ). This inhibition is mediated by a poxvirus-encoded serin protease inhibitor or serpin, first identified in cowpox virus as cytokine response modifier A (crmA) or SPI-2 ( http://jvi.asm.org/cgi/content/full/75/18/8353#B19 - 19 ). All orthopoxviruses encode serpins with SPI-1, -2, or -3 homology between Ect, cowpox virus (CPV), VV, rabbitpox virus (RPV), and variola virus of 92 to 97% ( http://jvi.asm.org/cgi/content/full/75/18/8353#B21 - 21 ). Yet despite such a high sequence conservation, VV, in contrast to Ect, CPV, and RPV, does not inhibit Tc cell-mediated lysis by either the exocytosis or the Fas cytolytic pathway ( http://jvi.asm.org/cgi/content/full/75/18/8353#B16 - 16 ). Thus, an IL-4-induced increase in Fas-mediated cytotoxicity will be effective in VV infections but not in Ect infections. This may explain the more dramatic increase in virulence of Ect ( http://jvi.asm.org/cgi/content/full/75/18/8353#B8 - 8 ) over VV ( http://jvi.asm.org/cgi/content/full/75/18/8353#B1 - 1 ) when IL-4 is expressed. Figure http://jvi.asm.org/cgi/content/full/75/18/8353#F1 - 1 dispicts this scenario in schematic form. One prediction would be that the Ect-IL-4 virus would be much less virulent if the SPI-2 gene were deleted. It is not known if variola virus infection alters target susceptibility to Tc cell lysis as is the case for Ect or CPV. Therefore it is also not known if insertion of IL-4 into variola virus would create a much more virulent virus for humans like what happened with Ect for mice and as such be a potential target as a biological warfare agent.

http://jvi.asm.org/cgi/content/full/75/18/8353/F1">
View larger version (27K):
http://jvi.asm.org/cgi/content/full/75/18/8353/F1 - [in this window]
href= - [in a new window]
 
FIG. 1.   Schematic representation of poxvirus-infected target cell killing by Tc cells. (Top) Antigen presentation, in the absence of IL-4, leads to activation of Tc cells capable of lysing target cells by both the perforin (exocytosis) and the Fas pathways. Target cells infected with Ect are lysed via the perforin pathway only, as SPI-2 of Ect inhibits killing via the Fas pathway. VV-infected targets are susceptible to lysis by both pathways, as VV-encoded SPI-2 does not interfere with the Fas pathway. (Bottom) Presentation of IL-4 during T-cell activation leads to the generation of Tc cells deficient in the perforin pathway ( http://jvi.asm.org/cgi/content/full/75/18/8353#B3 - 3 ). VV-infected targets can be lysed via the Fas pathway. Ect-infected targets are refractory to Tc cell attack.

Thus, available evidence fully predicted that (i) Ect-IL-4 recombinant virus would be more virulent and that antibody responses would not be enhanced and that (ii) increased virulence is in part also due to a switch by Tc cells from exocytosis-mediated cytolytic mechanisms to Fas-mediated killing, which is not executable in Ect-infected cells.


    FOOTNOTES

* Corresponding author. Mailing address: Division of Immunology and Cell Biology, John Curtin School of Medical Research, The Australian National University, P.O. Box 334, Canberra City, ACT 2601, Australia. Phone: 61261254392. Fax: 61262486271. E-mail: mailto:arno.mullbacher@anu.edu.au - arno.mullbacher@anu.edu.au < =text/> mailto:%27 + u + %27@%27 + d + %27 - ' + u + '@' + d + '<\/a>'//--> .


    REFERENCES
http://jvi.asm.org/cgi/content/full/75/18/8353#Top"> Top
http://jvi.asm.org/cgi/content/full/75/18/8353#Text"> Text
References

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2. Andrew, M. E., B. E. H. Coupar, and D. B. Boyle. 1989. Use of recombinant vaccinia viruses to study MHC class 1-restricted recognition and lymphokine function, p. 47-56. In R. V. Blanden (ed.), Immunology of virus diseases. Brolga Press, Curtin, Australia.
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11. Müllbacher, A., R. Wallich, R. W. Moyer, and M. M. Simon. 1999. Poxvirus encoded serpins do not prevent cytolytic T cell mediated recovery from primary infections. J. Immunol. 162:7315-7321 http://jvi.asm.org/cgi/ijlink?linkType=ABST&journalCode=jimmunol&resid=162/12/7315 - [Abstract/Free Full Text] .
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17. Simon, M. M., P. Waring, M. Lobigs, A. N., T. Tran, R. Tha Hla, S. Chin, and A. Müllbacher. 2000. Cytotoxic T cells specifically induce fas on target cells, thereby facilitating exocytosis-independent induction of apoptosis. J. Immunol. 165:3663-3672 http://jvi.asm.org/cgi/ijlink?linkType=ABST&journalCode=jimmunol&resid=165/7/3663 - [Abstract/Free Full Text] .
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20. Villacres, M. C., and C. C. Bergmann. 1999. Enhanced cytotoxic T cell activity in IL-4-deficient mice. J. Immunol. 162:2663-2670 http://jvi.asm.org/cgi/ijlink?linkType=ABST&journalCode=jimmunol&resid=162/5/2663 - [Abstract/Free Full Text] .
21. Wallich, R., M. M. Simon, and A. Müllbacher. 2001. Virulence of mousepox virus is independent of serpin-mediated control of cellular cytotoxicity. Viral Immunol. 14:71-81 http://jvi.asm.org/cgi/external_ref?access_num=10.1089/08828240151061428&link_type=DOI - [CrossRef] http://jvi.asm.org/cgi/external_ref?access_num=11270598&link_type=MED - [Medline] .


Journal of Virology, September 2001, p. 8353-8355, Vol. 75, No. 18
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.18.8353-8355.2001
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This article has been cited by other articles: ( http://jvi.asm.org/cgi/external_ref?access_num=http://jvi.asm.org/cgi/content/full/75/18/8353&link_type=GOOGLESCHOLAR - Search Google Scholar for Other Citing Articles )

  • Murata, K., Lechmann, M., Qiao, M., Gunji, T., Alter, H. J., Liang, T. J. (2003). Immunization with hepatitis C virus-like particles protects mice from recombinant hepatitis C virus-vaccinia infection. Proc. Natl. Acad. Sci. U. S. A. 100: 6753-6758 http://www.pnas.org/cgi/content/abstract/100/11/6753 - [Abstract] http://www.pnas.org/cgi/content/full/100/11/6753 - [Full Text]  
  • Mullbacher, A., Lobigs, M., Hla, R. T., Tran, T., Stehle, T., Simon, M. M. (2002). Antigen-Dependent Release of IFN-{gamma} by Cytotoxic T Cells Up-Regulates Fas on Target Cells and Facilitates Exocytosis-Independent Specific Target Cell Lysis. J Immunol 169: 145-150 http://www.jimmunol.org/cgi/content/abstract/169/1/145 - [Abstract] http://www.jimmunol.org/cgi/content/full/169/1/145 - [Full Text]  



Posted By: Guests
Date Posted: March 29 2006 at 2:45pm
Guys you dont get flu in a 'vile', I think MaryK meant a phile but anyway, viuses do not live outside of a host cell structure. They are parasitic not cultured.

HD


Posted By: Guests
Date Posted: March 29 2006 at 2:49pm
Harp,  actually, in Americaneese... it's vial.
 
BTW: H5N1 is an exceptionally hardy virus outside of a host.  Up to 48 hours on a non-porous surface in average temps.  And fed and nurtured, it can be kept going quite handily.
 
SZ


Posted By: Guests
Date Posted: March 29 2006 at 2:58pm
Right I knew there was something not quite right about the spelling. I was totally wrong as well with phile!!!!!

On a non-porous surface, etc. It still has to be in a host I beleive. Bird cell, etc. That's not that difficult though as they excreet a lot of those.
I dont beleive they exist as an entity in there own right for any time at all but I guess that's a mute point if you get the Bf from touching something inorganic.

I still think it's a chicken licken story.

HD
    


Posted By: Guests
Date Posted: March 29 2006 at 3:06pm
He SZ it's phial in the UK. Isn't Life Great!


Posted By: Fla_Medic
Date Posted: March 29 2006 at 3:11pm
HD, the virus needs a host cell to replicate, but it can certainly remain viable outside of a host cell for quite some time. 

While our demented chap was probably brandishing a vial of apple juice, it would certainly be possible to create a medium for the virus to survive in.  Blood, or Blood plasma comes to mind.




Posted By: sweets
Date Posted: March 29 2006 at 3:33pm

<img src=" http://www.exaltedshrimp.com/blob.jpg - http://www.exaltedshrimp.com/blob.jpg ">

 



-------------
I'm not a ribbering Jidiot!
www.exaltedshrimp.com


Posted By: Falcon
Date Posted: March 29 2006 at 3:37pm
one blood drop is all it takes really.
 
this is disturbing just hope it doesn't work


-------------
I look at the stars and wonder what it would be like to touch them.


Posted By: Guests
Date Posted: March 29 2006 at 4:10pm
MaryK,
 
As a former law enforcement officer this nutball made my hair stand on end.  If you felt uneasy around this kook - good for you.
 
Sadly, I was not surprised at the response of law enforcement.  It's all too complex for most of them.   .357 magnum is a big number for many of them.  That's why they switched to 9mm.
 
Next time you might try using one or two sylable words.  Like 'Bird' and 'Flu'.  If they look intelligent, try combining the two words into a term,  like 'Bird Flu'.
 
Good Luck


Posted By: maryk
Date Posted: March 30 2006 at 7:22am

I got no where with the terrorist task force, they sent me back to my local pd. So instead of going for that humiliating experience again i went to the wife of a police officer i work with. She called her husband. This is what he said he found out/ concluded from the event.

I went to the store in my uniform. I was alone, the guy must have recognized me from my work where he had over head my conversations about the BF. The guy is an Jerk and basically was making fun of me/ scaring me.


Posted By: Guests
Date Posted: March 30 2006 at 7:26am
A lesson for me and anyone else interested in viruses.

I am eternally grateful for this good person having these web pages. the animations are really informative. Will I eventually understand viruses?????

WEB PAGE FOR DR. KAISER'S MICROBIOLOGY COURSE
(BIOL 230)
THE COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS

Viruses are infectious agents with both living and nonliving characteristics.

1. Living characteristics of viruses

a. They reproduce at a fantastic rate, but only in living host cells.

b. They can mutate.

2. Nonliving characteristics of viruses

a. They are acellular, that is, they contain no cytoplasm or cellular organelles.

b. They carry out no metabolism on their own and must replicate using the host cell's metabolic machinery. In other words, viruses don't grow and divide. Instead, new viral components are synthesized and assembled within the infected host cell.

c. They possess DNA or RNA but never both.

Viruses that infect animal cells replicate by means of what is called the productive life cycle (def). Some viruses, such as HIV and the herpes viruses are able to become latent in certain cell types. A few viruses increase the risk of certain cancers.

We will now look at the life cycles of viruses that infect animal cells.

The Productive Life Cycle of Animal Viruses

Although there is great variation among the animal viruses, a generalized productive life cycle consists of the following steps:

a. Attachment or adsorption

Adsorption (def) (see Fig. 1A and Fig. 1B) involves the binding of attachment sites on the viral surface with receptor sites on the host cell cytoplasmic membrane.

For a virus to infect a host cell, that cell must have receptors for the virus on its surface and also be capable of supporting viral replication. These host cell receptors are normal surface molecules involved in routine cellular function, but since a portion of a molecule on the viral surface resembles the chemical shape of the body's molecule that would normally bind to the receptor, the virus is able to attach to the host cell's surface. For example, most human rhinoviruses that cause the common cold bind to intercellular adhesion molecules (ICAM-1) found on cells of the nasal epithelium. These ICAM-1 molecules are used normally for the recruitment of leukocytes into the respiratory tract. On the other hand, HIV adsorbs to CD4 molecules and chemokine receptors found on the surface of human T4-lymphocytes and macrophages (discussed later in this section). CD4 molecules are normally involved in immune recognition while chemokine receptors play a role in initiating inflammation and recruiting leukocytes.

Animation showing adsorption of an enveloped virus.
Animation showing adsorption of a naked virus.
b. Penetration

1. Enveloped viruses (def) enter the host cell in one of two ways:

a. In some cases, the viral envelope may fuse with the host cell cytoplasmic membrane and the nucleocapsid (def) is released into the cytoplasm (see Figs. 2A, Fig. 2B and Fig. 2C).

b. Usually they enter by endocytosis whereby the host cell cytoplasmic membrane invaginates and pinches off, placing the virus in an endocytic vesicle (see Fig. 3A, Fig. 3B, and Fig. 3C).

2. Naked viruses (def) enter the cell in one of two ways:

a. In many cases, interaction between the viral capsid and the host cell cytoplasmic membrane causes a rearrangement of capsid proteins allowing the viral nucleic acid to pass through the membrane into the cytoplasm (see Fig. 4A, Fig. 4B, and Fig. 4C).

b. The virus may enter by endocytosis whereby the host cell cytoplasmic membrane invaginates and pinches off, placing the virus in an endocytic vesicle (see Fig. 5A, Fig. 5B, and Fig. 5C).

c. uncoating

Uncoating is the release of the viral genome (def) from the remainder of the virus.

1. With enveloped viruses, the viral envelope is first removed either by fusing with the cytoplasmic membrane during penetration (see Fig. 2D and Fig. 2E) or fusing with the membrane of the endocytic vesicle after endocytosis (see Fig. 7A, Fig. 7B, and Fig. 7C). The viral capsid is then enzymatically removed and the viral genome is released.

Animation showing penetration and uncoating of an enveloped virus entering by fusion.
Animation showing penetration and uncoating of an enveloped virus entering by endocytosis.
2. In the case of naked viruses entering by fusion, interaction between the viral capsid (def) and the host cell cytoplasmic membrane (see Fig. 8) causes a rearrangement of capsid proteins allowing the viral nucleic acid to pass through the membrane into the cytoplasm. With naked viruses entering by endocytosis, the endocytic vesicle and the viral capsid are enzymatically removed and the viral nucleic acid is released into the host cell's cytoplasm (see Fig. 9A, Fig. 9B, and Fig. 9C).

Animation showing penetration and uncoating of a naked virus entering by fusion.
Animation showing penetration and uncoating of a naked virus entering by endocytosis.
Uncoating begins the eclipse period (def), the period during which no intact virions can be detected within the cell.

d. Replication

The viral genome directs the host cell's metabolic machinery (ribosomes, tRNA, nutrients, energy, enzymes, etc.) to synthesize viral enzymes and viral parts. The viral genome is transcribed into viral mRNA (see Fig. 10) that goes to the host cell's ribosomes where it is translated into viral structural proteins and viral enzymes. (Viral genetics will be discussed in Unit 4.) During the early phase of replication, the viral genome replicates thousands of times. During the late phase of replication, viral structural proteins (capsid and matrix proteins, envelope glycoproteins, etc.) and the enzymes involved in maturation are produced. Also during this time, viral proteins and glycoproteins coded by the viral genome are incorporated into the host cell's membranes (see Fig. 11). During the uncoating and replication stages the virus is not infectious.

For More Information: Transcription from Unit 4
For More Information: Translation from Unit 4
For More Information: Viral Genetics from Unit 4


e. Maturation

During maturation, the capsid is assembled around the genome (see Fig. 12A and Fig. 12B).

Animation showing replication and maturation of an enveloped virus.
Animation showing replication and maturation of a naked virus.


f. Release

1. With naked viruses, the infected cell usually disintegrates and the virions are released (see Fig. 13).

Animation showing release of a naked virus by cell disintegration.
2. With enveloped viruses, the host cell may or may not be lysed. The viruses obtain their envelopes from host cell membranes by budding. As mentioned above, prior to budding, viral proteins and glycoproteins are incorporated into the host cell's membranes. During budding the host cell membrane with incorporated viral proteins and glycoproteins evaginates and pinches off to form the viral envelope. Budding occurs either at the outer cytoplasmic membrane, the nuclear membrane, or at the membranes of the Golgi apparatus. Viruses obtaining their envelope from the cytoplasmic membrane are released during the budding process (see Fig. 14A and Fig. 14B). Those obtaining their envelopes from the membranes of the nucleus or the Golgi apparatus are then released by exocytosis via transport vesicles (see Fig. 15A and Fig. 15B).

Animation showing release of an enveloped virus by budding.
Animation showing release of an enveloped virus by exocytosis.
Some viruses, capable of causing cell fusion, may be transported from one cell to adjacent cells without being released, that is, they are transmitted by cell-to-cell contact whereby an infected cell fuses with an uninfected cell (see Fig. 16A, Fig. 16B, and Fig. 16C).

g. Reinfection

As many as 10,000 to 50,000 animal viruses may be produced by a single infected host cell.
    


Posted By: Guests
Date Posted: March 30 2006 at 7:37am
Does H5N1 use the budding type of release then?


Posted By: araywood
Date Posted: March 30 2006 at 8:00am

If he so much as even gets near you again file a restraining order. I am sure a judge would not find this situation funny at all.  Thats all you can do. Document any sighting of him. Number 1 rule of survival is never play the victim take the offense. Find his name out. Access the county clerk office (internet) pull all his background records. Name search him on the internet you want to find out all you can. Call his mother talk to her on the phone. This technique scares the Sh** out of most predators.

THATS ME.......Being niceBig smile
Usually I like to do deal with a situation like this with out right violence
it is something they seem to understand better.


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NO NEWS IS WHO NEWS



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